Valletta sounded a warning to their premier rivals with an 8-2 demolition of Hamrun Spartans.
Michael Mifsud scored a double hat-trick with four of his goals coming in the opening quarter hour.
The win leaves City on top of the premiership.
In the English Championship Huddersfield moved to second spot following their 3-1 win at Blackpool.
Real Madrid picked up their first away win of the season winning 2-nil at Rayo Vallecano in a match that had been postponed for twenty four hours after vandals damaged floodlights. The win lifts Real to seventh in La Liga but they still trail their arch rivals Barcelona by eight points.
In Italy Fiorentina take on Serie A leaders Juventus in a bid to halt the defending champions four match winning start to the season.
Tonight Manchester City task on fellow premier side Aston Villa in the third round of the League Cup.
Both sides have won only one of their last four league games.
Chelsea are home to Wolverhampton Wanderers for their third round tie and although the London club should start as favourites, they could have a tough match against a wolves side who are improving in the championship.
Everton face a battle away to Leeds, West Ham could be tested by the visit of Wigan and Sunderland will be expected to be too strong for Milton Keynes.
Around Europe, leaders Bayern Munich take on Wolfsburg in the Bundesliga while second placed Frankfurt are home to Borussia Dortmund.
Celtic play Raith Rovers in the third round of the Scottish League Cup.